Regulatory
Part:BBa_K101018:Design
Designed by: Karl Petersen Group: iGEM08_Minnesota (2008-10-28)
MioC Promoter (regulating tetR)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal SpeI site found at 314 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 314
Illegal NotI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal SpeI site found at 314 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 314 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Site directed mutagenesis was used to create a Spe1 restriction site.
Source
The MioC promoter is from K-12 and the tetR is the part BBa_I15040.